How to Use
SubMitoPred process only protein sequence file in fasta format. User have option to cut and paste in the text box or directly upload the sequence file. The limits of SubMitoPred is upto 25 sequences at a time. If user submit more than 25 sequences, SubMitoPred predict only first 25 sequences. Following figure 1 is an example showing how to submit a query in SubMitoPred.
SVM Threshold Value
User also have the option to set the threshold. If user select high threshold, the number of prediction will be low but rate of false positive prediction will also be low. If the user select low threshold, the number of prediction will be high and the false positive prediction will also be high.
Example Sequence
>sp|Q16891|MIC60_HUMAN MICOS complex subunit MIC60 OS=Homo sapiens GN=IMMT PE=1 SV=1
MLRACQLSGVTAAAQSCLCGKFVLRPLRPCRRYSTSGSSGLTTGKIAGAGLLFVGGGIGG
TILYAKWDSHFRESVEKTIPYSDKLFEMVLGPAAYNVPLPKKSIQSGPLKISSVSEVMKE
SKQPASQLQKQKGDTPASATAPTEAAQIISAAGDTLSVPAPAVQPEESLKTDHPEIGEGK
PTPALSEEASSSSIRERPPEEVAARLAQQEKQEQVKIESLAKSLEDALRQTASVTLQAIA
AQNAAVQAVNAHSNILKAAMDNSEIAGEKKSAQWRTVEGALKERRKAVDEAADALLKAKE
ELEKMKSVIENAKKKEVAGAKPHITAAEGKLHNMIVDLDNVVKKVQAAQSEAKVVSQYHE
LVVQARDDFKRELDSITPEVLPGWKGMSVSDLADKLSTDDLNSLIAHAHRRIDQLNRELA
EQKATEKQHITLALEKQKLEEKRAFDSAVAKALEHHRSEIQAEQDRKIEEVRDAMENEMR
TQLRRQAAAHTDHLRDVLRVQEQELKSEFEQNLSEKLSEQELQFRRLSQEQVDNFTLDIN
TAYARLRGIEQAVQSHAVAEEEARKAHQLWLSVEALKYSMKTSSAETPTIPLGSAVEAIK
ANCSDNEFTQALTAAIPPESLTRGVYSEETLRARFYAVQKLARRVAMIDETRNSLYQYFL
SYLQSLLLFPPQQLKPPPELCPEDINTFKLLSYASYCIEHGDLELAAKFVNQLKGESRRV
AQDWLKEARMTLETKQIVEILTAYASAVGIGTTQVQPE
>sp|P85799|PROH2_APIME Prohormone-2 OS=Apis mellifera PE=1 SV=1
MMCDWVWLLLTLCSLLMIVQSLPTNLAEDTKKTEQTMRPKSKRAQEMLMFGNQQNHQPEN
NPSSSYSSTAEKRTLAASGLGGLKAALIEEEKPSRSNTLNNAFYDRKNYDYGAVNELGYE
IPQVWDNSPYSRYYTNEDRRKRSEKSAVASGSSTTIKPSTTSFQSPTSTQQSVQTQVKRN
VPIYQEPRFKRELDIDPEDVLTLLSLWENERRKRNWHKYMNEEYENVDDEDNLLEEEDSR
NIIPWMDSSVYPPRHYSLDSLSPSDIGIIRTHPSSYYEQYENQYGQQYDTSQYGSPQYGL
VYPQQTYYSAPEKRFMISRKRSQAYDPYSNAAQFQLSSQSRGYPYQHRLVY
>sp|Q8IVP5|FUND1_HUMAN FUN14 domain-containing protein 1 OS=Homo sapiens GN=FUNDC1 PE=1 SV=1
MATRNPPPQDYESDDDSYEVLDLTEYARRHQWWNRVFGHSSGPMVEKYSVATQIVMGGVT
GWCAGFLFQKVGKLAATAVGGGFLLLQIASHSGYVQIDWKRVEKDVNKAKRQIKKRANKA
APEINNLIEEATEFIKQNIVISSGFVGGFLLGLAS
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Figure 1. SubMitoPred submission page showing how to insert input sequences.
Prediction Result
In response to the submitted protein sequence(s), SubMitoPred generates the prediction result in tabular form. We also provide a job number, if user have any query regarding their result, he/she can contact us. Here is a sample of prediction done in response to query sequences (Figure 2).
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Figure 2. Screenshot of SubMitoPred prediction result.